Quantitative Measurement of Immunoglobulins and Free Light Chains Using Mass Spectrometry

Quantitative measurement of immunoglobulins and free light chains using mass spectrometry.

Serum free light chain (sFLC) assays are well established in the diagnosis and monitoring of plasma cell disorders. However, current FLC immunoassays are subject to several analytical issues, which results in a lack of harmonized results. To facilitate sFLC standardization, we investigated the strengths and limitations of mass spectrometry as a novel technological platform for sFLC quantificati...

[Clinical value of the blood measurement of the free light chains of immunoglobulins].

Monoclonal free light chains are found in the serum and urine of patients with B-cell proliferative disorders, including multiple myeloma. Measuring free light chains in serum is useful for the diagnosis and monitoring of free light chains diseases. Moreover it could be interesting in the monitoring of treated multiple myeloma with complete immunoglobulin and of monoclonal gammapathy of undeter...

Radioimmunoassay of free light chains of immunoglobulins in urine.

We describe radioimmunoassays for kappa and lambda light chains of immunoglobulins in urine. The assays, which involve antisera to free light chains, were sufficiently sensitive to measure the light chains in unconcentrated urine from healthy subjects. The usefulness of the assays in clinical practice is illustrated by measurements of light chain excretion by patients, including serial studies ...

Free light chains of immunoglobulins: clinical laboratory analysis.

The increased sensitivity of immunofixation electrophoresis (IFE) over prior electrophoretic methods has led to renewed interest in the study of free light chains. Here, we discuss problems associated with the identification of monoclonal free light chains (Bence Jones proteins) in urine. Besides reviewing the nature of the sample specimens and the assays themselves, we discuss the physiology, ...

Measurement of free light chains in urine.